Intracellular movement of two mannose 6-phosphate receptors: return to the Golgi apparatus.
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چکیده
منابع مشابه
Intracellular movement of two mannose 6-phosphate receptors: return to the Golgi apparatus
We have used Chinese hamster ovary (CHO) cells and a murine lymphoma cell line to study the recycling of the 215-kD and the 46-kD mannose 6-phosphate receptors to various regions of the Golgi to determine the site where the receptors first encounter newly synthesized lysosomal enzymes. For assessing return to the trans-most Golgi compartments containing sialyltransferase (trans-cisternae and tr...
متن کاملAn ELISA method to quantify the mannose 6-phosphate receptors.
Mannose 6-phosphate receptor proteins mediate transport of lysosomal enzymes to lysosomes in eukaryotes. Two receptors designated as MPR 300 and MPR 46 based on their apparent molecular mass have been well studied from human and bovine liver. In humans, it has been shown that the receptors are present in different concentrations in different tissues. In the present study, MPR 300 and MPR 46 wer...
متن کاملMannose-6-phosphate receptors for lysosomal enzymes cycle between the Golgi complex and endosomes
We have examined the distribution of mannose-6-phosphate (Man6P) receptors (215 kD) for lysosomal enzymes in cultured Clone 9 hepatocytes at various times after the addition or removal of lysosomotropic weak bases (chloroquine or NH4Cl). Our previous studies demonstrated that after treatment with these agents, Man6P receptors are depleted from their sorting site in the Golgi complex and accumul...
متن کاملTail-specific antibodies that block return of 46,000 M(r) mannose 6- phosphate receptor to the trans-Golgi network
Recycling of 46,000 M(r) mannose 6-phosphate receptor (MPR 46) was investigated by microinjection of Fab fragments against small epitopes within the cytoplasmic domain of the receptor. Fab fragments against the peptide 43-47 (Ala-Tyr-Arg-Gly-Val) efficiently blocked return of MPR 46 to the TGN. Antibody-induced redistribution resulted in accumulation of MPR 46 within an endosomal compartment, f...
متن کاملTail-specific Antibodies That Block Return of 46,000 Mr Mannose 6-Phosphate Receptor to the trans-Golgi Network
Recycling of 46,000 Mr mannose 6-phosphate receptor (MPR 46) was investigated by microinjection of Fab fragments against small epitopes within the cytoplasmic domain of the receptor. Fab fragments against the peptide 43-47 (Ala-Tyr-Arg-Gly-Val) efticiently blocked return of MPR 46 to the TGN. Antibody-induced redistribution resulted in accumulation of MPR 46 within an endosomal compartment, fro...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1988
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.106.3.617